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Scientists developed enzymes with remote control

The new system's scheme. Credit: ITMO University     Scientists have developed a method to enhance the activity of enzymes by using…

The new system’s scheme. Credit: ITMO University

Scientists have developed a method to enhance the activity of enzymes by using radio frequency radiation. The method requires a special complex of enzymes and magnetic nanoparticles. The particles can adsorb radio emission and convert it to heat, resulting in the acceleration of enzymatic processes by more than four times. De methode kan worden gebruikt om radio-gecontroleerde biochemische systemen en aanpassing metabolisme in levende organismen. The results are published in ACS Biomaterials Science & Engineering .

Enzymes are involved in a variety of reactions in living organisms, and their effectiveness depends on a variety of conditions. Selv om enzymaktivitet er normalt kontrolleret kemisk, forskere fra ITMO University har vist at dette kan ske fjernt ved hjælp af fysiske metoder som radiofrekvensområder.

To make radio-controlled enzymes, the scientists synthesized a special complex in which an enzyme is enclosed in a rigid porous framework of magnetite nanoparticles. Whenever the radio field is applied, the nanoparticles adsorb radio emission and heat up, adding additional energy to the enzyme and resulting in the enzymatic reaction rate acceleration. An experiment conducted on a model enzyme, carbonic anhydrase, demonstrated that the reaction rate can be increased by more than four times.

“There were very few studies out there that explored enzyme manipulation through the radio waves. Typically, these enzymes change the conformation at high temperatures and then stop working. But placed within the rigid framework of nanoparticles, the enzyme is stabilized from structural rearrangements as the nanoparticles mechanically restrict the enzyme mobility, “says Andrey Drozdov, member of ITMO University’s SCAMT Laboratory.

There are two key parameters among the advantages of the radio emission used in the work. Such radio waves can easily pass through tissues, and they are harmless. Således, ved anvendelse av radiofrekvensområdet kan forskere styre aktiviteten af ​​enzymer i kroppen og justere cell metabolisme. In de nabije toekomst zullen de wetenschappers plannen om deze methode te testen op andere enzymen in een poging om de vitale activiteit van bacteriën of cellen te beïnvloeden.

Aangezien dit onderwerp veel van potentie heeft, zal verdere werkzaamheid zich concentreren op het gebruik van de techniek met other enzymes, as well as in living cells. For example, it is still unclear whether it is possible to make bacteria or cells divide more often, or to stop their division.

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More information:
Yulia I. Andreeva et al., Enzymatic Nanocomposites with Radio Frequency Field Modulated Activity, ACS Biomaterials Science & Engineering (2018). DOI: 10.1021 / acrobatic materials.8b00838

Provided By:
ITMO University

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